Image Collection using an Auto Data Acquisition System and An Application to Ice embedded Ribosome
نویسندگان
چکیده
Single particle analysis is a tomographic method to reconstruct a three-dimensional structure from a set of two-dimensional projected images acquired by a transmission electron microscope (TEM). This method is applied to reconstruct biological samples such as proteins or viruses. The micrographs of cryo biological samples are obtained by using a minimum dose system (MDS), because these samples are sensitive to incident electrons. The low dose leads to bad signal noise ratio (SNR) of the particle images picked from the micrographs. In order to obtain a high contrast image of the particles, similar images picked from the micrographs are classified and merged by hierarchical ascendant classification, which requires many micrographs. Consequently, many images of the particle are necessary to reconstruct a three-dimensional structure by the single particles analysis.
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